What is the reason behind the reddish hue of DMEM?
The Phenol Red utilized in Dulbecco's Modified Eagle's Medium (DMEM) serves as a vital pH indicator. When the pH of the solution is 6.8, indicating an acidic environment (below 7), it assumes a yellow hue. Conversely, at a pH of 8.2, signifying alkalinity (above 8), it transforms into a red color. The transition to a yellow tint suggests a reduction in pH, often attributed to alterations in CO2 levels during the freezing process. Posted on 27th October 2020.
Is the A549 cell line a suspension or adherent culture?
The A549 cell line is propagated in a comprehensive medium, specifically Dulbecco's MEM enriched with 10% FBS. These cells have the capability to proliferate as adherent cells or in suspension within an in vitro environment. Notably, the A549 cell line exhibits a rapid growth rate, with a cell doubling time ranging from 24 to 40 hours.
Could you elaborate on the determination of the A549 cell's superficial dimensions?
(3) In regards to the individual A549 cell, the comprehensive measurement revealed that the mitochondrial volume and surface were 61.91 μm³ and 1001.67 μm² respectively. Additionally, the lamellar bodies exhibited a total volume of 76.82 μm³ and a surface area of 428.68 μm². Lastly, the lysosomes displayed a total volume of 21.69 μm³ and a surface area of 212.04 μm².
What are the steps involved in the preservation of hela cells through freezing?
Process
Process the trypsinization of 10 flasks utilizing 2ml of Tryp solution. ...
Disperse the cells into a suitable medium (approximately 8ml for 3 flasks)
Collect the cell suspensions in a 50ml centrifuge tube.
Spin down the mixture at 1500 rpm for 5 minutes.
Discard the supernatant.
Re-suspend the cell pellet in 20ml of a freezing medium, which is a combination of regular growth medium and 5% sterile DMSO.
Divide the suspension into 20 individual Cryo tubes, each containing 1ml.
Additional steps...•
Could you elaborate on the distinction between the hams f10 and the F12?
R.G. Ham formulated Ham's F-12, a nutrient blend tailored specifically for serum-free cultivation of Chinese Hamster Ovary (CHO) single-cells. Unlike Ham's F-10, this medium boasts a higher zinc concentration, enhancing the plating efficiency and reproducibility of CHO cells.a549 cell line media
What type of cells underwent cultivation in the DMEM medium?
Culturing cells in DMEM has resulted in successful propagation of various types, including primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. Additionally, cell lines like HeLa, 293, Cos-7, and PC-12 have also thrived in this medium. Gibco's DMEM is formulated with the utmost quality, consistency, and precision to cater to your mammalian cell culture needs.psn1 cell line
What is the rationale behind utilizing isopropanol in the MTT assay?
The presence of HCl in the tissue culture medium transforms the phenol red into a yellow hue, ensuring no interference with the MTT formazan assay. Furthermore, the addition of isopropanol facilitates the dissolution of formazan, resulting in a consistent blue solution optimal for absorbance evaluation.sw1573 cell line
Could you elaborate on the significance of DMEM and FBS?
The DMEM Complete Medium comprises of DMEM high glucose medium, enriched with 2mM L-glut, sodium pyruvate, and 10% FBS sourced from the United States. This medium serves as a ready-to-use, sterile formula, ideally suited for the propagation of diverse mammalian cell types, ranging from primary mouse and chicken cells to various normal and transformed cell lines.
What role does phenol red play in the functionality of DMEM?
Phenol red, primarily utilized as a pH indicator in cell culture, exhibits estrogenic properties stemming from its structural resemblance to nonsteroidal estrogens and its capacity to stimulate cell growth in certain cell types. This observation was made on 9 May 2020.
What solvent is suitable for MTT?
The decrease in MTT levels is observed specifically in metabolically active cells, with the extent of this reduction serving as an indicator of cellular viability. To quantify the formation of insoluble formazan crystals, an organic solvent, typically dimethyl sulfoxide, is employed. These purple-hued crystals are then assessed using a spectrophotometer for accurate measurement.
By:Helena